Hayashi T, Itagaki H, Fukuda T, Tamura U, Sato Y, Suzuki Y
Shiseido Research Center, Yokohama, Japan.
Biol Pharm Bull. 1995 Apr;18(4):540-3. doi: 10.1248/bpb.18.540.
The properties of hemoglobin denaturation caused by two anionic surfactants (sodium dodecylsulfate and sodium lauroylmethyltaurate) were examined using spectrophotometry (multi-plate reader), circular dichroism (CD) and high-performance liquid chromatography (HPLC), and their denaturating action on hemoglobin and sorption to it were examined by various methods. Correlation and factorial analyses were applied to the experimental data and the following results were obtained: (1) High correlations were found among sorption, denaturation and alpha-helix content and the random structure of hemoglobin. (2) The alpha-helix of hemoglobin is randomized as the surfactants denature hemoglobin. (3) Factorial analysis indicates that there are two factors involved in hemoglobin denaturation, one related to the destruction of the alpha-helix and the other related to a change in the beta-structure. These two factors are related to change in the environment around the heme group. (4) Destruction of the alpha-helix seems to be one of the causes of the eye irritation produced by anionic surfactants.
使用分光光度法（酶标仪）、圆二色光谱（CD）和高效液相色谱（HPLC）研究了两种阴离子表面活性剂（十二烷基硫酸钠和月桂酰甲基牛磺酸钠）引起的血红蛋白变性特性，并通过多种方法研究了它们对血红蛋白的变性作用及其对血红蛋白的吸附。对实验数据进行了相关性和因子分析，得到以下结果：（1）吸附、变性与血红蛋白的α-螺旋含量及无规结构之间存在高度相关性。（2）随着表面活性剂使血红蛋白变性，血红蛋白的α-螺旋变得无序。（3）因子分析表明，血红蛋白变性涉及两个因素，一个与α-螺旋的破坏有关，另一个与β-结构的变化有关。这两个因素与血红素基团周围环境的变化有关。（4）α-螺旋的破坏似乎是阴离子表面活性剂引起眼睛刺激的原因之一。
