Schlatt S, Arslan M, Weinbauer G F, Behre H M, Nieschlag E
Institute of Reproductive Medicine, University Münster, Germany.
Eur J Endocrinol. 1995 Aug;133(2):235-47. doi: 10.1530/eje.0.1330235.
Four groups(N = 3 per group) of juvenile rhesus monkeys (Macaca mulatta, 14-20 months old) received either vehicle or highly purified human follicle-stimulating hormone (FSH; 10 IU kg-1 day-1), human chorionic gonadotropin (hCG; 250 IU every alternate day) or both hormones for a period of 4 weeks. Testicular volume and weight increased more than twofold after single and more than sixfold after combined hormone treatment. Serum and intratesticular testosterone were at supraphysiological levels in hCG-treated animals and rose even more after combined treatment; a minor elevation of intratesticular testosterone was also observed after FSH treatment. Serum inhibin was elevated after hCG or FSH treatment and increased more than twofold during the first 3 weeks of combined treatment. Semiquantitative analysis of cell numbers showed a statistically non-significant increase in Sertoli cells and Ad- and Ap-spermatogonia after single hormone treatment. Combined treatment induced a further increase in the number of spermatogonia. Leydig cells were only encountered after hCG treatment; their number was more than threefold higher after combined treatment compared with hCG alone. Follicle-stimulating hormone stimulated Sertoli cell and Ap spermatogonia proliferation but did not induce morphological differentiation of Sertoli cells, peritubular cells or Leydig cells. Human CG treatment, however, induced Sertoli cell proliferation and morphological differentiation. It had effects on spermatogonial proliferation but induced differentiation of peritubular cells. Combined treatment initiated the greatest morphological and functional differentiation of Sertoli cells, peritubular cells, Leydig cells and spermatogonia. Flow cytometric analysis confirms an increase of mitotically active cells. The observations show that FSH and testosterone can induce Sertoli cell proliferation. Morphological differentiation of Sertoli cells may be mediated indirectly by environmental and paracrine stimuli released from peritubular cells, whose differentiation is androgen dependent. Leydig cells are stimulated mainly by hCG. Our present and previous data lead us to propose that FSH contributes to the final number and activity of Leydig cells, which secrete immunoreactive inhibin in response to hCG.(ABSTRACT TRUNCATED AT 400 WORDS)
将四组（每组N = 3）幼年恒河猴（猕猴，14 - 20个月大）分别给予赋形剂或高纯度人促卵泡激素（FSH；10 IU·kg⁻¹·天⁻¹）、人绒毛膜促性腺激素（hCG；每隔日250 IU）或两种激素，为期4周。单次激素治疗后睾丸体积和重量增加两倍以上，联合激素治疗后增加六倍以上。hCG治疗动物的血清和睾丸内睾酮处于超生理水平，联合治疗后进一步升高；FSH治疗后睾丸内睾酮也有轻微升高。hCG或FSH治疗后血清抑制素升高，联合治疗前3周内升高两倍以上。细胞数量的半定量分析显示，单次激素治疗后支持细胞以及Ad-和Ap-精原细胞数量有统计学上无显著意义的增加。联合治疗使精原细胞数量进一步增加。仅在hCG治疗后发现有间质细胞；联合治疗后的间质细胞数量比单独使用hCG时高出三倍以上。促卵泡激素刺激支持细胞和Ap精原细胞增殖，但未诱导支持细胞、睾丸周细胞或间质细胞的形态分化。然而，人绒毛膜促性腺激素治疗诱导了支持细胞增殖和形态分化。它对精原细胞增殖有影响，但诱导了睾丸周细胞分化。联合治疗引发了支持细胞、睾丸周细胞、间质细胞和精原细胞最大程度的形态和功能分化。流式细胞术分析证实有丝分裂活跃细胞增加。这些观察结果表明，FSH和睾酮可诱导支持细胞增殖。支持细胞的形态分化可能由睾丸周细胞释放的环境和旁分泌刺激间接介导，睾丸周细胞的分化依赖雄激素。间质细胞主要受hCG刺激。我们目前和之前的数据使我们提出，FSH有助于间质细胞的最终数量和活性，间质细胞在hCG作用下分泌免疫反应性抑制素。（摘要截短至400字）
