Gonschior P, Goetz A E, Huehns T Y, Höfling B
Medical Department I, Klinikum Grosshadern, University of Munich, Germany.
Coron Artery Dis. 1995 Apr;6(4):329-34. doi: 10.1097/00019501-199504000-00010.
Local drug delivery using a new 5F catheter with six small needles is described. The needles can be extended laterally into vascular tissue for drug deposition.
A fluorescent indicator (Photofrin) was injected with a new local drug delivery device into porcine carotid arteries. The vessels were explanted 15, 30, 60 min and 14 days after local drug delivery. Vascular segments were analyzed using semi-quantitative measurement of fluorescence (calculated in relation to a standard, 100% representing the maximum fluorescence achieved by systemic intravenous application of Photofrin).
Maximum fluorescence was found in adventitia (15 min: 374%; 30 min: 388%; 60 min: 251%). In intimal tissue, the detected fluorescence was 107% after 15 min, 294% after 30 min, and 25% after 60 min. Media fluorescence was lower (15 min: 151%, 30 min: 102%, 60 min: 55%). No systemic drug content was measured. Fourteen days after local drug delivery, 15% of maximal fluorescence was still found in media but no adverse tissue hyperplasia was observed.
These experiments demonstrate that high-dose perivascular local drug delivery is feasible and allows prolonged and selective application of drugs in a vessel segment without side effects.
描述了一种使用带有六根小针的新型5F导管进行局部药物递送的方法。这些针可以横向延伸至血管组织以进行药物沉积。
使用一种新型局部药物递送装置将荧光指示剂（卟吩姆钠）注入猪的颈动脉。在局部药物递送后15分钟、30分钟、60分钟和14天取出血管。使用荧光的半定量测量（相对于标准计算，100%代表通过全身静脉注射卟吩姆钠获得的最大荧光）对血管段进行分析。
在外膜中发现最大荧光（15分钟：374%；30分钟：388%；60分钟：251%）。在内膜组织中，15分钟后检测到的荧光为107%，30分钟后为294%，60分钟后为25%。中膜荧光较低（15分钟：151%，30分钟：102%，60分钟：55%）。未检测到全身药物含量。局部药物递送14天后，中膜中仍发现最大荧光的15%，但未观察到不良组织增生。
这些实验表明，高剂量血管周围局部药物递送是可行的，并且可以在血管段中长时间选择性地应用药物而无副作用。
