Zhang M, Guo M, Jin H
Departments of Pathophysiology and Pathology, Shanghai Medical University.
Zhonghua Yi Xue Za Zhi. 1995 May;75(5):273-5, 319.
The pure mesangial cells (MsC) were isolated from the culture of rat glomeruli. After identification, they were divided into heparin-treatment group and control group. It was found that heparin profoundly inhibited the growth of MsC with 75.9% inhibitory rate by means of direct counting MsC. MTT method proved that the inhibitory effect of heparin on MsC was dose-dependent. Electronmicroscopic observation revealed dilated endoplasmic reticulum and Golgi vesicles, more secondary lysosomes, prominent myelin figures and decreased microfilaments in the cytoplasm of the MsC exposed to heparin. Immunocytochemical studies showed that the amounts of FN and LM in the cytoplasm of MsC decreased after heparin treatment. Immunocytochemical investigation also confirmed that heparin could inhibit the expression of nuclear oncogene c-fos and c-jun in the MsC stimulated by PMA. The significance and possible mechanism of inhibitory effect of heparin on MsC were discussed.
从大鼠肾小球培养物中分离出纯系膜细胞（MsC）。鉴定后，将其分为肝素处理组和对照组。通过直接计数MsC发现，肝素能显著抑制MsC的生长，抑制率达75.9%。MTT法证明肝素对MsC的抑制作用呈剂量依赖性。电镜观察显示，暴露于肝素的MsC细胞质中内质网和高尔基体囊泡扩张，次级溶酶体增多，髓鞘样结构突出，微丝减少。免疫细胞化学研究表明，肝素处理后MsC细胞质中FN和LM的量减少。免疫细胞化学研究还证实，肝素可抑制PMA刺激的MsC中核癌基因c-fos和c-jun的表达。并对肝素抑制MsC作用的意义及可能机制进行了讨论。
