人类胶原结合蛋白2基因（CBP2）的分离、特性鉴定及染色体定位

Isolation, characterization and chromosomal assignment of human colligin-2 gene (CBP2).

作者信息

Ikegawa S, Sudo K, Okui K, Nakamura Y

机构信息

Department of Biochemistry, Cancer Institute, Tokyo, Japan.

出版信息

Cytogenet Cell Genet. 1995;71(2):182-6. doi: 10.1159/000134103.

DOI:10.1159/000134103

PMID:7656593

Abstract

Colligin, a collagen-binding glycoprotein localized to endoplasmic reticulum, belongs to the serpin superfamily. Colligin has been identified and cloned in a number of species including mouse, rat, chicken, and human. We have isolated and characterized a full-length human cDNA clone that encodes a 418-amino-acid peptide that is highly homologous (97% identity) to the previously reported human colligin gene, and have named this new member of the colligin family human colligin-2. The expression of the colligin-2 gene (CBP2) is ubiquitous among all normal human tissues except for brain and peripheral leukocytes. We have mapped this novel gene to chromosomes 11q13.5 by fluorescent in situ hybridization (FISH) using a cosmid clone containing the entire coding sequence and the 3' non-coding sequence.

摘要

胶原结合蛋白（Colligin）是一种定位于内质网的胶原结合糖蛋白，属于丝氨酸蛋白酶抑制剂超家族。在包括小鼠、大鼠、鸡和人类在内的多个物种中已鉴定并克隆出胶原结合蛋白。我们分离并鉴定了一个全长人类cDNA克隆，该克隆编码一个418个氨基酸的肽，与先前报道的人类胶原结合蛋白基因高度同源（97%同一性），并将这个胶原结合蛋白家族的新成员命名为人类胶原结合蛋白-2（human colligin-2）。除了脑和外周白细胞外，胶原结合蛋白-2基因（CBP2）在所有正常人体组织中普遍表达。我们使用包含整个编码序列和3'非编码序列的黏粒克隆，通过荧光原位杂交（FISH）将这个新基因定位到染色体11q13.5上。