Wada H, Muro K, Hirata T, Yodoi J, Hitomi S
Department of Thoracic Surgery, Kyoto University, Japan.
Chest. 1995 Sep;108(3):810-4. doi: 10.1378/chest.108.3.810.
Thioredoxin (TRX) production has been shown to be induced by a variety of stresses. In this study, we examined TRX expression in lung tissues after canine lung transplantation to determine whether it could be induced by allogenic stimulations and could be used to diagnose early rejection. Thirty-five adult mongrel dogs were used in all. In group A, 24 dogs underwent allotransplantation of the left lung with no immunosuppressant and were sacrificed at various times; 5 were sacrificed on postoperative day (POD) 1; 5 on POD 2; 7 on POD 3; and 7 on POD 5. In group B, 5 donor right lungs were used for negative control. In group C, 3 dogs underwent autotransplantation. In group D, 8 dogs underwent allotransplantation of the left lung with optimal immunosuppression and were sacrificed at POD 28. Lung tissues were stained with anti-TRX antibody, and the TRX high-producer (TRXh) cells in a randomly chosen field were counted as the index of TRX expression. In group A, the number of TRXh cells were as follows: 1.68 +/- 1.14 in grade 0; 4.87 +/- 1.07 in grade 1; 10.42 +/- 4.24 in grade 2; 27.34 +/- 17.96 in grade 3; and 50.90 +/- 17.36 in grade 4. In group B, the number of TRXh cells was 1.82 +/- 1.01. There was a significant difference between each rejection grade in group A and group B (p < 0.01), and we could observe TRXh cells in the early stage of rejection. These results suggest that analysis of TRXh cells in lung tissues may be useful in the early diagnosis of rejection.
硫氧还蛋白（TRX）的产生已被证明可由多种应激诱导。在本研究中，我们检测了犬肺移植后肺组织中TRX的表达，以确定其是否可由同种异体刺激诱导，并能否用于早期排斥反应的诊断。总共使用了35只成年杂种犬。A组中，24只犬在未使用免疫抑制剂的情况下接受左肺同种异体移植，并在不同时间处死；5只在术后第1天（POD 1）处死；5只在POD 2处死；7只在POD 3处死；7只在POD 5处死。B组中，5个供体右肺用作阴性对照。C组中，3只犬接受自体移植。D组中，8只犬在最佳免疫抑制下接受左肺同种异体移植，并在POD 28处死。用抗TRX抗体对肺组织进行染色，并将随机选择视野中的TRX高产细胞（TRXh）数量作为TRX表达的指标。A组中，TRXh细胞数量如下：0级为1.68±1.14；1级为4.87±1.07；2级为10.42±4.24；3级为27.34±17.96；4级为50.90±17.36。B组中，TRXh细胞数量为1.82±1.01。A组和B组各排斥等级之间存在显著差异（p<0.01），且我们能在排斥反应早期观察到TRXh细胞。这些结果表明，分析肺组织中的TRXh细胞可能有助于早期诊断排斥反应。
