Li Y, Hui Y, Zhou J
Department of Ophthalmology, Xijing Hospital, 4th Medical Military University, Xi'an.
Zhonghua Yan Ke Za Zhi. 1995 Mar;31(2):105-8.
To investigate the role of vimentin gene in cataractogenesis, 12.7 Kb chicken vimentin genes were microinjected into the male pronuclei of 530 fertilized mouse eggs. 453 injected embryos were transferred into the oviducts of 18 pseudopregnant recipient females, of which 9 pregnant mice gave births to 37 offspring mice. The integration and expression of exogenous gene in the offsprings were analyzed by Southern and Northern blot hybridizations. It is shown that the E2 offspring was a transgenic mouse in which the chicken vimentin gene was integrated in its genome and efficiently expressed in mRNA level in its lens. In the human senile cataract lens, Bam-HI-restricted vimentin fragments displayed a stronger hybridization signal than that of the control lens in Southern blot analysis.
为研究波形蛋白基因在白内障发生过程中的作用，将12.7kb的鸡波形蛋白基因显微注射到530枚受精小鼠卵的雄原核中。453枚注射后的胚胎被移植到18只假孕受体雌性小鼠的输卵管中，其中9只怀孕小鼠产下37只后代小鼠。通过Southern和Northern印迹杂交分析后代中外源基因的整合和表达情况。结果显示，E2后代是一只转基因小鼠，鸡波形蛋白基因整合到其基因组中，并在晶状体的mRNA水平上高效表达。在人类老年性白内障晶状体中，Southern印迹分析显示，经Bam-HI酶切的波形蛋白片段比对照晶状体显示出更强的杂交信号。
