Chen Y, Dong J, Li C
Department of Pathology, First Military Medical University, Guangzhou.
Zhonghua Zhong Liu Za Zhi. 1995 Jan;17(1):16-9.
Quantitative DNA dot blot hybridization and immunohistochemical method were employed to detect amplification and overexpression of proto-oncogene c-erbB2 in 101 paraffin-embedded breast cancers. The results showed that c-erbB2 was amplified and overexpressed in 34.7% and 23.8% of examined cases respectively. The overexpression of c-erbB2 was correlated with, but not always paralled to the gene amplification. The moderate to high-level overexpression of c-erbB2 detected by immunohistochemistry was significantly associated with the size of the tumour and the number of lymph nodes involved. No differences were found in the alteratio of c-erbB2 gene and its protein expression between primary and metastatic breast cancers indicating that the genotypic and phenotypic changes of c-erbB2 occured prior to and maintained in the process of metastasis of breast cancer.
采用定量DNA斑点杂交和免疫组化方法检测101例石蜡包埋乳腺癌中原癌基因c-erbB2的扩增及过表达情况。结果显示，在所检测病例中，c-erbB2基因扩增和过表达分别占34.7%和23.8%。c-erbB2的过表达与基因扩增相关，但并不总是平行。免疫组化检测到的c-erbB2中至高水平过表达与肿瘤大小及受累淋巴结数目显著相关。原发性和转移性乳腺癌之间c-erbB2基因及其蛋白表达的改变无差异，表明c-erbB2的基因型和表型变化发生于乳腺癌转移之前，并在转移过程中持续存在。
