Tan D, Gu Z, Wang H
First Department of Internal Medicine. Air Force 466 Hospital, Beijing.
Zhonghua Zhong Liu Za Zhi. 1995 Mar;17(2):93-6.
In this study, the effect was studied of estradiol (E2) and tamoxifen on the growth of a human gastric adenocarcinoma SGC-7901 cell line in vitro. Estrogen receptor as revealed by immunohistochemical staining was positive when added at various concentrations. E2 could significantly stimulate cell growth. The effect was most marked at concentration of 0.1 mumol/L. The cell doubling time was reduced, the plating efficiency was increased and c-myc oncogene expression was enhanced. Flow cytometric analysis indicated that cell] proliferation index was greater in the E2-treated than in the control. All these effects caused by E2 could be partially inhibited by the estrogen receptor inhibitor tamoxifen.
本研究探讨了雌二醇（E2）和他莫昔芬对人胃腺癌SGC - 7901细胞系体外生长的影响。免疫组织化学染色显示，添加不同浓度的雌激素受体呈阳性。E2能显著刺激细胞生长，在0.1μmol/L浓度时作用最为明显，可使细胞倍增时间缩短，接种效率提高，c - myc癌基因表达增强。流式细胞术分析表明，E2处理组的细胞增殖指数高于对照组。E2所引起的所有这些效应均可被雌激素受体抑制剂他莫昔芬部分抑制。
