Rentsch D, Laloi M, Rouhara I, Schmelzer E, Delrot S, Frommer W B
Institut für Genbiologische Forschung, Berlin, Germany.
FEBS Lett. 1995 Aug 21;370(3):264-8. doi: 10.1016/0014-5793(95)00853-2.
Hterologous complementation of yeast mutants has enabled the isolation of genes encoding several families of amino acid transporters. Among them, NTR1 codes for a membrane protein with weak histidine transport activity. However, at the sequence level, NTR1 is related to rather non-specific oligopeptide transporters from a variety of species including Arabidopsis and to the Arabidopsis nitrate transporter CHL1. A yeast mutant deficient in oligopeptide transport was constructed allowing to show that NTR1 functions as a high affinity, low specificity peptide transporter. In siliques NTR1-expression is restricted to the embryo, implicating a role in the nourishment of the developing seed.
酵母突变体的异源互补使得编码几个氨基酸转运蛋白家族的基因得以分离。其中,NTR1编码一种具有弱组氨酸转运活性的膜蛋白。然而,在序列水平上,NTR1与包括拟南芥在内的多种物种中相当非特异性的寡肽转运蛋白以及拟南芥硝酸盐转运蛋白CHL1相关。构建了一个寡肽转运缺陷的酵母突变体,从而表明NTR1作为一种高亲和力、低特异性的肽转运蛋白发挥作用。在角果中,NTR1的表达仅限于胚胎,这表明其在发育中的种子营养供应中发挥作用。