Neuberger J, Wallace L, Joplin R, Hubscher S
Liver Unit, Queen Elizabeth Hospital, Birmingham, UK.
Hepatology. 1995 Sep;22(3):798-801.
We have examined the distribution of the E2 component of pyruvate dehydrogenase complex in the liver of patients with native primary biliary cirrhosis (PBC), and after transplantation, using affinity-purified anti-E2 antibodies. In the posttransplantation group, we studied biopsy specimens from patients grafted for conditions other than PBC (n = 6) and those grafted for PBC with (n = 6) and without (n = 5) histological features suggestive of PBC recurrence. Features suggestive of PBC recurrence included portal tract granulomas, bile duct damage, ductopenia, and lymphoid aggregates. In the native liver from patients with PBC, there was increased staining of E2 on the biliary epithelial cells compared with hepatocytes, as previously described. However, in liver biopsy specimens from patients after transplantation, the pattern of staining of E2 was similar to that of normal, control liver in all three groups studied. These findings suggest that E2 overexpression on bile duct cells may not be important in the perpetuation of the bile duct damage in PCB, that expression in the allograft may be modified by immunosuppression, or that PBC does not recur in the allograft.
我们使用亲和纯化的抗E2抗体，研究了原发性胆汁性肝硬化（PBC）患者及其肝移植后肝脏中丙酮酸脱氢酶复合体E2成分的分布情况。在移植后组中，我们研究了因非PBC疾病接受移植患者的活检标本（n = 6），以及因PBC接受移植且有（n = 6）和无（n = 5）提示PBC复发组织学特征患者的活检标本。提示PBC复发的特征包括门管区肉芽肿、胆管损伤、胆管减少和淋巴样聚集。正如之前所描述的，在PBC患者的天然肝脏中，与肝细胞相比，胆管上皮细胞上E2的染色增加。然而，在移植后患者的肝脏活检标本中，在所有研究的三组中，E2的染色模式与正常对照肝脏相似。这些发现表明，胆管细胞上E2的过表达在PBC胆管损伤的持续存在中可能并不重要，同种异体移植物中的表达可能因免疫抑制而改变，或者PBC在同种异体移植物中不会复发。
