Isolation and properties of a conditionally lethal bacteriophage lambda mutated in the chi region.

A thermosensitive lambda phage mutant was isolated which can grow at high temperature only in the presence of the lambda chi gene product supplied in trans. This mutation tn was mapped within the chi region, and lambda tn phage expressed the pRoR-OP operon only poorly at high temperature. Effects of the tn mutation on expression of other operons were also examined and compared with those observed with cro27 or some tof mutations.