[无载体¹²⁵I标记及测定尖吻蝮蛇毒]

[Radiolabelling and assay of Chinese agkistrodon acutus venom with carrier-free Na 125I].

作者信息

Gong Y, Deng C, Li S, Li L, Guan J

机构信息

Department of Internal Medicine, Guangzhou Medical College.

出版信息

Hua Xi Yi Ke Da Xue Xue Bao. 1995 Mar;26(1):116-9.

PMID:7657328

Abstract

Chinese agkistroden acutus venom (CAAV) was radiolabelled with carrier-free Na 125I by the method of Iodogen. The specific activity and radiochemical purity for radiolabelled products were 4236.5 x 10(10) Bq/mmol and 98%, respectively. Each CAAV molecule carried 0.52 125I atom. Physical and chemical characterization of radiolabelled CAAV was similar to unradiolabelled CAAV. Binding analysis showed that 125I-CAAV was bound to platelet in a saturable manner. Binding sites per platelet were 13,255 +/- 6292/platelet. The dissociation constant (Kd) was 3.2 +/- 0.69 x 10(-10) mol/L. These results are similar to binding sites of other snake venom on platelet. The investigation showed that radiolabelled CAAV made by our laboratory was useful for radioligand binding assay.

摘要

采用碘代法用无载体的Na 125I对中国尖吻蝮蛇毒（CAAV）进行放射性标记。放射性标记产物的比活度和放射化学纯度分别为4236.5×10(10) Bq/mmol和98%。每个CAAV分子携带0.52个125I原子。放射性标记的CAAV的物理化学特性与未标记的CAAV相似。结合分析表明，125I-CAAV以饱和方式与血小板结合。每个血小板的结合位点为13255±6292/血小板。解离常数（Kd）为3.2±0.69×10(-10) mol/L。这些结果与其他蛇毒在血小板上的结合位点相似。研究表明，我们实验室制备的放射性标记的CAAV可用于放射性配体结合测定。