Hilderson H, Berghen P, De Graaf D C, Claerebout E, Vercruysse J
Department of Parasitology, Faculty of Veterinary Medicine, University of Gent, Merelbeke, Belgium.
Int J Parasitol. 1995 Jun;25(6):757-60. doi: 10.1016/0020-7519(94)00196-u.
Excretory-secretory and somatic antigens of Ostertagia ostertagi fourth stage larvae, emulsified with complete Freund's adjuvant, were intraperitoneally administered to calves on three occasions. Two weeks after the last immunisation all calves were infected with a single dose of 130,000 O. ostertagi third stage larvae. All animals were necropsied 25 days after infection. The immunisation procedure resulted in IgG1 and IgG2 antibodies, however no protective immunity was induced as O. ostertagi worm burdens and worm length were similar in all groups.
将奥斯特他加线虫第四期幼虫的排泄分泌抗原和虫体抗原与完全弗氏佐剂乳化后，分三次对犊牛进行腹腔注射。最后一次免疫两周后，所有犊牛均单次感染130,000条奥斯特他加线虫第三期幼虫。感染后25天对所有动物进行剖检。免疫程序产生了IgG1和IgG2抗体，但由于所有组的奥斯特他加线虫虫负荷和虫体长度相似，未诱导出保护性免疫。
