Misteli T, Warren G
Cell Biology Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
J Cell Biol. 1995 Sep;130(5):1027-39. doi: 10.1083/jcb.130.5.1027.
Golgi stacks were previously shown to be converted into tubular networks when incubated in mitotic cytosol depleted of the coatomer subunit of COP I coats (Misteli and Warren, 1994). Similar, though smaller, networks are now shown to be an early intermediate on the Golgi fragmentation pathway both in vitro and in vivo. Their appearance mirrors the disappearance of Golgi cisternae and at their peak they constitute 35% of total Golgi membrane. They are consumed by two pathways, the first involving the budding of COP I-coated vesicles described previously (Misteli and Warren, 1994). The second involves a COP I-independent mechanism that leads eventually to a vesicle fraction that is larger in size and more heterogeneous than that produced by the COP I-mechanism. We suggest that both pathways operate concurrently at the onset of mitotic fragmentation. The COP I-independent pathway converts cisternae into tubular networks that then fragment. The COP I-dependent pathway partially consumes first the cisternae at the beginning of the incubation and then the tubular networks that form from them.
先前的研究表明，当高尔基体堆叠在去除了COP I衣被的外被体亚基的有丝分裂胞质溶胶中孵育时，会转化为管状网络（米斯特利和沃伦，1994年）。现在发现，类似但较小的网络在体外和体内都是高尔基体碎片化途径中的早期中间体。它们的出现与高尔基体潴泡的消失相对应，在高峰期它们占高尔基体总膜的35%。它们通过两条途径被消耗，第一条途径涉及先前描述的COP I包被囊泡的出芽（米斯特利和沃伦，1994年）。第二条途径涉及一种不依赖COP I的机制，最终导致形成一个囊泡部分，其大小比由COP I机制产生的囊泡更大且更具异质性。我们认为这两条途径在有丝分裂碎片化开始时同时起作用。不依赖COP I的途径将潴泡转化为管状网络，然后这些网络碎片化。依赖COP I的途径首先在孵育开始时部分消耗潴泡，然后消耗由它们形成的管状网络。
