Ghishan F K, Knobel S, Barnard J A, Breyer M
Department of Pediatrics, Vanderbilt Medical School, Nashville, Tennessee 37232, USA.
J Membr Biol. 1995 Apr;144(3):267-71. doi: 10.1007/BF00236840.
We have recently cloned a novel Na+/H+ exchanger (NHE-2) from rat small intestinal cDNA library. The present report characterizes the expression of (NHE-2) mRNA in the rat small intestine, stomach, and kidney using Northern blot analysis and in situ hybridization techniques. NHE-2 mRNA was detected in the surface epithelium of the stomach, villus but not crypt epithelial cells of the small intestine and in the distal convoluted, but not in the proximal tubules of the kidney. This distribution suggests a functional role in the transport of sodium across the apical membrane of the gastrointestinal tract and the kidney.
我们最近从大鼠小肠cDNA文库中克隆出一种新型的钠氢交换体（NHE-2）。本报告利用Northern印迹分析和原位杂交技术，对NHE-2 mRNA在大鼠小肠、胃和肾脏中的表达进行了表征。在胃的表面上皮、小肠绒毛而非隐窝上皮细胞以及肾脏的远曲小管而非近端小管中检测到了NHE-2 mRNA。这种分布表明其在胃肠道和肾脏顶膜钠转运中发挥功能性作用。
