Araki T, Shimizu M, Yoshio H, Ino H, Mabuchi H, Takeda R
2nd Department of Internal Medicine, Kanazawa University School of Medicine, Japan.
Jpn Circ J. 1995 Apr;59(4):213-8. doi: 10.1253/jcj.59.213.
To examine the effects of angiotensin-converting enzyme (ACE) inhibitor and aldosterone antagonist on myocardial collagen in the cardiomyopathic hamster, the collagen concentration was measured by determining the hydroxyproline concentration, and the ratio of type I to type III collagen (type I/III ratio) was measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Five-week-old Bio14.6 cardiomyopathic hamsters were treated with the ACE inhibitor captopril (20 mg/kg per day) or the aldosterone antagonist K-canrenoate (20 mg/kg per day) in drinking water for 20 weeks, and the collagen concentration and type I/III ratio at 25 weeks were compared with those in 25-week-old untreated Bio14.6 and normal F1b hamsters. The collagen concentration markedly increased and the type I/III ratio significantly decreased (ie, type III collagen dominant) in untreated Bio14.6 compared with F1b at 25 weeks. Captopril and K-canrenoate treatment significantly reduced the collagen concentration and reversed the changes in the type I/III ratio in cardiomyopathic hamster. These results suggest that ACE inhibitor and aldosterone antagonist improve myocardial collagen in the cardiomyopathic hamster, not only quantitatively but also qualitatively, and that the mechanism of this improvement may be related to the cardiac renin-angiotensin-aldosterone system.
为研究血管紧张素转换酶（ACE）抑制剂和醛固酮拮抗剂对心肌病仓鼠心肌胶原的影响，通过测定羟脯氨酸浓度来测量胶原浓度，并采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法测量Ⅰ型与Ⅲ型胶原的比例（Ⅰ/Ⅲ比例）。将5周龄的Bio14.6心肌病仓鼠用饮用水中的ACE抑制剂卡托普利（每天20毫克/千克）或醛固酮拮抗剂钾-坎利酸钾（每天20毫克/千克）治疗20周，并将25周时的胶原浓度和Ⅰ/Ⅲ比例与25周龄未治疗的Bio14.6和正常F1b仓鼠进行比较。与25周龄的F1b相比，未治疗的Bio14.6中胶原浓度显著增加，Ⅰ/Ⅲ比例显著降低（即Ⅲ型胶原占主导）。卡托普利和钾-坎利酸钾治疗显著降低了心肌病仓鼠的胶原浓度，并逆转了Ⅰ/Ⅲ比例的变化。这些结果表明，ACE抑制剂和醛固酮拮抗剂不仅在数量上而且在质量上改善了心肌病仓鼠的心肌胶原，并且这种改善机制可能与心脏肾素-血管紧张素-醛固酮系统有关。
