使用63Ni2+螯合法定量寡组氨酸融合蛋白。 - Suppr

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超能文献

Quantitation of oligohistidine fusion proteins using 63Ni2+ chelation.

作者信息

Ravagnani A, Arst H N

机构信息

Department of Infectious Diseases and Bacteriology, Royal Postgraduate Medical School, London, UK.

出版信息

Nucleic Acids Res. 1995 Aug 11;23(15):3069-70. doi: 10.1093/nar/23.15.3069.

DOI:10.1093/nar/23.15.3069

PMID:7659532

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307152/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c77/307152/0d5e63b4c1ec/nar00015-0277-a.jpg

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Measurement of protein using bicinchoninic acid.使用二辛可宁酸测定蛋白质。

Anal Biochem. 1985 Oct;150(1):76-85. doi: 10.1016/0003-2697(85)90442-7.

New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues.对含有相邻组氨酸残基的蛋白质和肽具有选择性的新型金属螯合吸附剂。

J Chromatogr. 1987 Dec 18;411:177-84. doi: 10.1016/s0021-9673(00)93969-4.

Detection and use of recombinant staphylococcal protein A fusion proteins to localize nucleic-acid-binding domains of proteins.重组葡萄球菌蛋白A融合蛋白的检测及用于定位蛋白质核酸结合结构域

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Protein blotting method for detection of nickel-binding proteins.用于检测镍结合蛋白的蛋白质印迹法。

Res Commun Chem Pathol Pharmacol. 1989 Sep;65(3):275-88.

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