Muñoz S, Lorca M, Muñoz P, Solari A
Special Programme for Research and Training in Tropical Diseases, SAREC (Swedish Agency for Research and Cooperation), DTI Universidad de Chile.
Rev Med Chil. 1994 Nov;122(11):1231-8.
Trypanosoma cruzi from 23 chronic and acute cases of Chagas disease were isolated and further characterized. They displayed a remarkable similarity, after typification by schizodeme analysis using restriction endonuclease Eco Ri. All T cruzi isolates belonged to the isoenzymatic Zymodeme 2 Bol. These findings were corroborated analyzing cases from families with demonstrated congenital transmission. The acute cases were newborns or offspring of infected mothers or patients with AIDS. All T cruzi strains isolated in these cases were very similar with almost identical schizodemes. Patients with AIDS had severe clinical manifestations whereas children with congenital infections had a great variety of clinical symptoms. In chronic cases, little clinical differences were observed for the different T cruzi strains. It is concluded that there is a low correlation between the T cruzi strain and the clinical prognosis of Chagas disease, being of greater relevance the host's genetic background and immune response.
从23例慢性和急性恰加斯病患者中分离出克氏锥虫，并对其进行了进一步鉴定。在用限制性内切酶Eco Ri进行裂殖体分析进行分型后，它们表现出显著的相似性。所有克氏锥虫分离株均属于同工酶酶型2 Bol。通过分析有先天性传播证据的家庭中的病例，证实了这些发现。急性病例为新生儿、感染母亲的后代或艾滋病患者。在这些病例中分离出的所有克氏锥虫菌株非常相似，裂殖体几乎相同。艾滋病患者有严重的临床表现，而先天性感染的儿童有各种各样的临床症状。在慢性病例中，不同克氏锥虫菌株的临床差异很小。得出的结论是，克氏锥虫菌株与恰加斯病的临床预后之间的相关性较低，宿主的遗传背景和免疫反应更为重要。
