Wendt M, Amtsberg G
Klinik für kleine Klauentiere, Tierärztlichen Hochschule Hannover.
Schweiz Arch Tierheilkd. 1995;137(4):129-36.
Determination of antibodies against Eubacterium suis (E. suis) in the serum of pigs was carried out by an indirect immunofluorescence test. Therefore 35 sows with urinary tract infections as well as 43 healthy control sows were investigated. E. suis could be detected in the urine of 19 sows. Serological results were compared with the bacteriological examinations. To reach a specificity of 100% for detection of animals actually infected with E. suis, titres from 1:16++ can be accepted as positive. Therefore sensitivity gave a level of 78.9%. Seroconversion and development of antibody titers were studied on 15 sows, which were experimentally infected with E. suis. Antibodies could be shown 3 weeks after infection at the earliest, they were preferably demonstrated in sows with a haemorrhagic cystitis. No correlation existed between the affection of the kidneys and the development and the level of titres respectively. Serological investigations can turn out negative in spite of E. suis infection, if immunological response still has not taken place or development of antibodies has failed to appear after short-time infection of the bladder.
通过间接免疫荧光试验对猪血清中抗猪优杆菌(E. suis)的抗体进行检测。因此,对35头患有尿路感染的母猪以及43头健康对照母猪进行了调查。在19头母猪的尿液中检测到了E. suis。将血清学结果与细菌学检查结果进行了比较。为使检测实际感染E. suis的动物的特异性达到100%,1:16++及以上的滴度可判定为阳性。因此,敏感性为78.9%。对15头经实验感染E. suis的母猪进行了血清转化和抗体滴度变化的研究。最早在感染后3周可检测到抗体,在患有出血性膀胱炎的母猪中更容易检测到。肾脏病变与抗体滴度的变化及水平之间均无相关性。如果免疫反应尚未发生,或者在膀胱短期感染后抗体尚未出现,尽管存在E. suis感染,血清学检测结果仍可能为阴性。