Yu D H, Wan L T, Lou Y Q
Department of Pharmacology, Beijing Medical University.
Yao Xue Xue Bao. 1995;30(4):286-90.
A method was developed for the determination of the novel antihypertensive drug naftopidil in biological samples by HPLC. Metoprolol was used as the internal standard. The analytical column was a stainless steel column filled with 10 micron C18 packing. The mobile phase was a mixture of methanol-acetonitrile-water-0.2 mol.L-1 HAc-0.2 mol.L-1 NaAc (50:45:5:0.9:0.1). Detection was performed at UV 232 nm. Biological samples can be well purified after two extractions with ether. The lowest detection limit was 5 ng.ml-1. The precision and accuracy within-day and day-to-day ranged from 3.17 to 10.88%. The mean recoveries were 79.35% to 95.72%. The results showed that this method was simple, sensitive and good enough to be used in pharmacokinetic study of naftopidil.
建立了一种采用高效液相色谱法测定生物样品中新型抗高血压药物萘哌地尔的方法。美托洛尔用作内标。分析柱为填充10微米C18填料的不锈钢柱。流动相为甲醇 - 乙腈 - 水 - 0.2 mol.L-1 HAc - 0.2 mol.L-1 NaAc（50:45:5:0.9:0.1）的混合物。在232 nm紫外波长下进行检测。生物样品经两次乙醚萃取后可得到良好纯化。最低检测限为5 ng.ml-1。日内和日间的精密度和准确度范围为3.17%至10.88%。平均回收率为79.35%至95.72%。结果表明，该方法简单、灵敏，足以用于萘哌地尔的药代动力学研究。
