Zhu C B, Li X Y, Zhu Y H, Xu S F
Department of Neurobiology, Shanghai Medical University, China.
Zhongguo Yao Li Xue Bao. 1995 May;16(3):201-4.
To study the expression of preproenkephalin (PPE) mRNA following electroacupuncture (EA) combined with droperidol (Dro), an antagonist of dopamine receptors.
The brains of Sprague-Dawley rats were sectioned after combination of EA with Dro and nonradioactive in situ hybridization histochemistry (ISHH) technic was used.
Ten hours after EA, the expression of PPE mRNA was enhanced; when EA was combined with Dro, the expression of PPE mRNA was further enhanced in many pain-modulation-related nuclei, such as caudate-putamen, accumbens, septal nucleus, diagonal band nucleus, amygdala, hypothalamus, periaqueductal gray (PAG), interpeduncular nucleus, substantia nigra, and the dorsal horn of spinal cord (layer I-II and III-IV).
Dro promoted EA's action on the PPE mRNA expression, which underlie the mechanisms of Dro potentiation on acupuncture analgesia.
研究电针（EA）联合多巴胺受体拮抗剂氟哌利多（Dro）后前脑啡肽原（PPE）mRNA的表达。
将EA与Dro联合应用于Sprague-Dawley大鼠后，取其脑切片，采用非放射性原位杂交组织化学（ISHH）技术。
EA后10小时，PPE mRNA表达增强；当EA与Dro联合应用时，在许多与疼痛调制相关的核团中，如尾状核-壳核、伏隔核、隔核、斜角带核、杏仁核、下丘脑、导水管周围灰质（PAG）、脚间核、黑质以及脊髓背角（Ⅰ-Ⅱ层和Ⅲ-Ⅳ层），PPE mRNA的表达进一步增强。
Dro促进了EA对PPE mRNA表达的作用，这是Dro增强针刺镇痛作用的机制基础。
