Cui X
Department of Physiology, Beijing Medical University.
Sheng Li Ke Xue Jin Zhan. 1995 Jul;26(3):230-2.
Using the techniques of immunohistochemistry, Northern blotting and in situ hybridization, we have found that electroacupuncture (2/15Hz) accelerated the expression of mRNA and protein synthesis of immediate early genes c-fos, c-jun as well as proenkephalin gene in rat central nervous system (CNS). Northern hybridization revealed that EA stimulation induced c-fos gene expression within 1-2 h, followed by PENK gene expression which peaked at 48h. Morphological study demonstrated that the sites of c-fos, c-jun and PENK mRNA expression and protein synthesis coincide with each other in the CNS. The results suggest that Fos/Jun may function as the transcription regulator of PENK gene.
运用免疫组织化学、Northern印迹法和原位杂交技术，我们发现电针（2/15Hz）可加速大鼠中枢神经系统（CNS）中即刻早期基因c-fos、c-jun以及前脑啡肽基因的mRNA表达和蛋白质合成。Northern杂交显示，电针刺激在1-2小时内诱导c-fos基因表达，随后是前脑啡肽基因表达，其在48小时达到峰值。形态学研究表明，CNS中c-fos、c-jun和前脑啡肽mRNA表达及蛋白质合成的位点相互吻合。结果提示，Fos/Jun可能作为前脑啡肽基因的转录调节因子发挥作用。
