吡喹酮诱导21日龄日本血吸虫体表损伤的扫描电镜观察
Scanning electron microscope observation on tegumental damage of 21-d-old Schistosoma japonicum induced by praziquantel.
作者信息
Xiao S H, Shen B G
机构信息
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.
出版信息
Zhongguo Yao Li Xue Bao. 1995 May;16(3):273-5.
AIM
To study the effect of praziquantel (Pra) on the tegument of 21-d-old schistosomula, mice infected with Schistosoma japonicum cercariae for 21 d were treated ig with Pra at a single dose.
METHOD
Groups of mice were killed at different intervals within 48 h, and the worms were collected by perfusion for scanning electron microscopic observation.
RESULTS
When the dose used was 300 mg.kg(-1), the worms showed mild or moderate swelling, fusion or even erosion and collapsed of the tegumental ridges, which was characterized by swelling of the discoid sensory structures. At higher dose of 500 mg.kg(-1), similar alterations in the worm surface were seen, but more extensive and serious. When Pra 500 mg.kg(-1) was given daily for 3 d, severe swelling, erosion and peeling of the tegument accompanied by the attachment of the host leukocytes on the damaged surface were seen.
CONCLUSION
Pra exhibited a direct killing effect on 21-d-old schistosomula.
目的
研究吡喹酮（Pra）对21日龄童虫体表的影响，对感染日本血吸虫尾蚴21天的小鼠进行单剂量Pra灌胃治疗。
方法
在48小时内不同时间点处死小鼠，通过灌注收集虫体进行扫描电子显微镜观察。
结果
当剂量为300mg·kg⁻¹时，虫体出现轻度或中度肿胀、融合，甚至体表嵴的侵蚀和塌陷，其特征为盘状感觉结构肿胀。在500mg·kg⁻¹的较高剂量下，虫体表面出现类似改变，但更广泛和严重。当每天给予500mg·kg⁻¹的Pra，持续3天时，可见体表严重肿胀、侵蚀和脱落，同时宿主白细胞附着于受损表面。
结论
Pra对21日龄童虫具有直接杀伤作用。
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