Osborne B I, Baker B
Plant Gene Expression Center, Albany, California, USA.
Curr Opin Cell Biol. 1995 Jun;7(3):406-13. doi: 10.1016/0955-0674(95)80097-2.
Transposons have been successfully exploited as insertional mutagens for the efficient identification and isolation of genes (transposon tagging) in many organisms. Plants are no exception. The maize Activator and Suppressor-mutator transposons function when transferred into heterologous plant species, and many different gene tagging systems have been developed. These systems have recently been used to clone novel and important genes, including disease resistance loci from Nicotiana tabacum, tomato and flax.
转座子已被成功用作插入诱变剂,用于在许多生物体中高效鉴定和分离基因(转座子标签法)。植物也不例外。玉米的激活子和抑制子突变转座子在转入异源植物物种时发挥作用,并且已经开发了许多不同的基因标签系统。这些系统最近已被用于克隆新的重要基因,包括来自烟草、番茄和亚麻的抗病基因座。