Kramerov A A, Arbatskiĭ N P, Rozovskiĭ Ia M, Pochechueva T I, Mikhaleva E A, Druzhinina T I, Gvozdev V A, Shibaev V N
Biokhimiia. 1995 May;60(5):805-12.
The glycoprotein from cultured cells of D. melanogaster, also detected in various insect tissues as a component of the extracellular matrix, was characterized as a mucin-type glycoprotein not yet described in invertebrates. This glycoprotein with an apparent molecular mass of approximately 90 kDa contains about 40% of carbohydrates, largely represented mainly by GalNAc and Gal; its polypeptide moiety is enriched with Thr, Ser, Pro and Gly. An analysis of oligosaccharides liberated by treatment of the glycoprotein with alkaline NaBH4 or O-glycanase (endo-alpha-N-acetylgalactosaminidase) revealed Gal(beta 1-3)GalNAc as the major type of the sugar chains. About half of the Thr + Ser residues in the glycoprotein were estimated as O-glycosidically linked with the disaccharide units.
在果蝇培养细胞中发现的糖蛋白，在各种昆虫组织中也作为细胞外基质的一个组分被检测到，其被鉴定为一种在无脊椎动物中尚未描述过的粘蛋白型糖蛋白。这种表观分子量约为90 kDa的糖蛋白含有约40%的碳水化合物，主要由GalNAc和Gal组成；其多肽部分富含苏氨酸（Thr）、丝氨酸（Ser）、脯氨酸（Pro）和甘氨酸（Gly）。用碱性NaBH4或O-聚糖酶（内切α-N-乙酰半乳糖胺酶）处理该糖蛋白后释放出的寡糖分析表明，Gal(β1-3)GalNAc是糖链的主要类型。据估计，该糖蛋白中约一半的Thr + Ser残基通过O-糖苷键与二糖单元相连。
