Exogenous gonadotropin administration affects the glycocalyx of rat endometrial epithelial cells during the period of implantation.

PURPOSE

Exogenous gonadotropins which cause superovulation are known to effect endometrial morphology, including the glycocalyx of surface epithelial cells. Certain of the carbohydrates in the glycocalyx of surface epithelial cells may be involved in the attachment and implantation of the blastocyst.

METHODS

The effect of exogenous gonadotropins on specific carbohydrates in the glycocalyx of the rat endometrium around the time of implantation was investigated. Lectin-avidin-biotin-ferritin cytochemistry was used to ascertain which carbohydrates were affected. The lectins soybean agglutinin, fucose binding protein and wheat germ agglutinin were used.

RESULTS

Statistically significantly less lectin was associated with the apical membrane of surface epithelial cells of animals following hyperstimulation than in noninjected pregnant animals.

CONCLUSIONS

The reduction in the carbohydrates contributes to a reduced receptivity of the endometrium for the blastocyst.