来自环孢素产生菌雪白丝枝霉的亲环蛋白基因的克隆与测序
Cloning and sequencing of a cyclophilin gene from the cyclosporin producer Tolypocladium niveum.
作者信息
Hornbogen T, Zocher R
机构信息
Institut für Biochemie und Molekulare Biologie, Technischen Universität Berlin, Germany.
出版信息
Biochem Mol Biol Int. 1995 May;36(1):169-76.
The gene of a 19 kDa cyclophilin of Tolypocladium niveum was isolated and sequenced. The open reading frame of 956 bp is interrupted by four introns of 76, 221, 58, and 61 bp size. 101 nucleotides upstream of the start codon ATG we found a putative promoter region containing a TATA and a CAAT box. The predicted amino acid sequence shows high identity to other cyclophilins, especially to the cyclophilin A family members of eucaryotic and procaryotic origin. Similar to the case of N. crassa we found a segment of 13 additional amino acid residues near the N-terminus which is not present in the other cyclophilins.
分离并测序了雪白丝枝霉19 kDa亲环蛋白的基因。956 bp的开放阅读框被4个大小分别为76、221、58和61 bp的内含子打断。在起始密码子ATG上游101个核苷酸处，我们发现了一个推定的启动子区域，其中包含一个TATA盒和一个CAAT盒。预测的氨基酸序列与其他亲环蛋白具有高度同源性，尤其是与真核和原核来源的亲环蛋白A家族成员。与粗糙脉孢菌的情况类似，我们在N端附近发现了一段额外的13个氨基酸残基片段，这在其他亲环蛋白中不存在。
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