Frigerio G, Pelham H R
Division of Cell Biology, MRC Laboratory of Molecular Biology, Cambridge, England.
J Mol Biol. 1993 Sep 5;233(1):183-8. doi: 10.1006/jmbi.1993.1497.
Cyclophilins are cyclosporin A sensitive peptidyl-prolyl cis-trans isomerases found in the cytoplasm of a wide range of species from Escherichia coli to man. Cyclophilin homologues are translocated into mitochondria, the endoplasmic reticulum and the bacterial periplasmic space. Here, the nucleotide sequence of a non-essential fifth Saccharomyces cerevisiae cyclophilin homologue encoded by chromosome XII is presented. As expected from its hydrophobic signal sequence and a hydrophilic carboxy terminus ending in the tetrapeptide HDEL, epitope-tagged cyclophilin D was found associated with the endoplasmic reticulum.
亲环蛋白是对环孢菌素A敏感的肽基脯氨酰顺反异构酶，存在于从大肠杆菌到人类等广泛物种的细胞质中。亲环蛋白同源物可转运至线粒体、内质网和细菌周质空间。本文给出了由十二号染色体编码的酿酒酵母非必需的第五种亲环蛋白同源物的核苷酸序列。正如根据其疏水信号序列和以四肽HDEL结尾的亲水羧基末端所预期的那样，发现表位标记的亲环蛋白D与内质网相关。
