从人血浆中对合成阿片肽和“抗阿片肽”进行高效液相色谱分离

High-performance liquid chromatographic resolution of synthetic opiate and "anti-opiate" peptides from human plasma.

作者信息

Partilla J S, You J, Rothman R B

机构信息

Clinical Psychopharmacology Section, National Institute on Drug Abuse, Baltimore, MD 21224, USA.

出版信息

J Chromatogr B Biomed Appl. 1995 May 5;667(1):49-56. doi: 10.1016/0378-4347(94)00594-u.

DOI:10.1016/0378-4347(94)00594-u

PMID:7663685

Abstract

An assay system using reversed-phase high-performance liquid chromatographic (HPLC) resolution of synthetic anti-opioid peptides (AOPs) and opioid peptides (OPs) was developed. Samples were diluted with trifluoroacetic acid, loaded onto Sep-Pak C18 cartridges, eluted, dried, and redissolved in ethanol-acetic acid-water. Retention-time consistency was established, and high levels of synthetic AOP and OP recovery, generally higher than 80%, were achieved. In a single HPLC run synthetic enkephalins, dynorphins, and beta-endorphins were separated even when extracted from human plasma using a volatile mobile phase which yielded fractions totally compatible with quantitation by radioimmunoassay. Combining the resolution of HPLC with the sensitivity of radioimmunoassay (RIA) may facilitate simultaneous measurement of numerous neuropeptides in body fluids such as plasma and cerebrospinal fluid.

摘要

开发了一种利用反相高效液相色谱（HPLC）分离合成抗阿片肽（AOPs）和阿片肽（OPs）的分析系统。样品用三氟乙酸稀释，加载到Sep-Pak C18柱上，洗脱、干燥，然后重新溶解于乙醇-乙酸-水。建立了保留时间的一致性，合成AOP和OP的回收率很高，通常高于80%。在一次HPLC运行中，即使使用挥发性流动相从人血浆中提取，合成脑啡肽、强啡肽和β-内啡肽也能被分离，得到的馏分完全适合用放射免疫测定法定量。将HPLC的分离能力与放射免疫测定法（RIA）的灵敏度相结合，可能有助于同时测量体液（如血浆和脑脊液）中的多种神经肽。