Skripal' I G, Onishchenko A M, Gavrilko L O
Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kiev.
Mikrobiol Z. 1995 Mar-Apr;57(2):31-40.
Monosaccharide composition of glycopolymers of five mollicutes (Mycoplasma pneumoniae FH, M. hominis PG 21, M. fermentans PG 18, Acholeplasma laidlawii PG 8 and A. laidlawii var. granulum 118) integrated with their membrane has been studied by means of nine plant lectins with certain carbohydrate specificity labelled with colloid gold. Monosaccharide composition of glycopolymers depends both on the mollicute species and on its capacity to evoke diseases in different microorganisms. Thus genetically relative A. laidlawii PG 8 (saprophytic microorganism) and A. laidlawii var. granulum 118 (an agent of pale-green dwarfness of cereals) have the ratio between N-acetyl-D. galactosamine and N-acetyl-D-glucosamine 2:1 and 1:2, respectively. Large amounts of such exotic sugar as L-fucose have been found in M. hominis PG 21, M. fermentans PG 18 and A. laidlawii PG 8. L-fucose plays the basic role in disguising cells of these mollicutes in human organism (as O(H)-group blood erythrocytes), which permits these mollicutes disseminating through the whole organism. The composition of extracellular glycopolymers of mollicutes is discussed to reveal their role in the processes of adhesion and specialization (organotropism) of these microorganisms in populating the corresponding organs and tissues of macroorganisms.
利用九种具有特定碳水化合物特异性且用胶体金标记的植物凝集素，研究了五种支原体（肺炎支原体FH、人型支原体PG 21、发酵支原体PG 18、莱氏无胆甾原体PG 8和莱氏无胆甾原体颗粒变种118）与细胞膜结合的糖聚合物的单糖组成。糖聚合物的单糖组成既取决于支原体种类，也取决于其在不同微生物中引发疾病的能力。因此，基因相关的莱氏无胆甾原体PG 8（腐生微生物）和莱氏无胆甾原体颗粒变种118（谷类植物浅绿色矮化病病原体）中，N-乙酰-D-半乳糖胺与N-乙酰-D-葡萄糖胺的比例分别为2:1和1:2。在人型支原体PG 21、发酵支原体PG 18和莱氏无胆甾原体PG 8中发现了大量诸如L-岩藻糖这样的外来糖。L-岩藻糖在人体中对这些支原体细胞起到伪装作用（如同O(H)血型的红细胞），这使得这些支原体能够在整个机体中扩散。本文讨论了支原体细胞外糖聚合物的组成，以揭示它们在这些微生物定殖于大型生物体相应器官和组织过程中的黏附及特异性（器官嗜性）作用。
