Rubin D A, Dores R M
Department of Biological Sciences, University of Denver, Colorado 80208, USA.
Mol Phylogenet Evol. 1995 Jun;4(2):129-38. doi: 10.1006/mpev.1995.1013.
In order to obtain a more resolute phylogeny of teleosts based on growth hormone (GH) sequences, phylogenetic analyses were performed in which deletions (gaps), which appear to be order specific, were upheld to maintain GH's structural information. Sequences were analyzed at 194 amino acid positions. In addition, the two closest genealogically related groups to the teleosts, Amia calva and Acipenser guldenstadti, were used as outgroups. Modified sequence alignments were also analyzed to determine clade stability. Analyses indicated, in the most parsimonious cladogram, that molecular and morphological relationships for the orders of fishes are congruent. With GH molecular sequence data it was possible to resolve all clades at the familial level. Analyses of the primary sequence data indicate that: (a) the halecomorphean and chondrostean GH sequences are the appropriate outgroups for generating the most parsimonious cladogram for teleosts; (b) proper alignment of teleost GH sequence by the inclusion of gaps is necessary for resolution of the Percomorpha; and (c) removal of sequence information by deleting improperly aligned sequence decreases the phylogenetic signal obtained.
为了基于生长激素（GH）序列获得更可靠的硬骨鱼系统发育树，进行了系统发育分析，其中保留了似乎具有顺序特异性的缺失（空位）以维持GH的结构信息。在194个氨基酸位置对序列进行了分析。此外，与硬骨鱼在系统发育上关系最密切的两个类群，即雀鳝和欧洲鳇，被用作外类群。还分析了修改后的序列比对以确定分支稳定性。分析表明，在最简约的分支图中，鱼类各目的分子和形态关系是一致的。利用GH分子序列数据可以在科级水平上解析所有分支。对一级序列数据的分析表明：（a）全骨鱼类和软骨硬鳞鱼类的GH序列是生成硬骨鱼最简约分支图的合适外类群；（b）通过纳入空位对硬骨鱼GH序列进行正确比对对于鲈形目的解析是必要的；（c）删除比对不当的序列会去除序列信息，从而降低获得的系统发育信号。
