在神经母细胞瘤细胞系NGP中鉴定出两个不同的源自12号染色体的扩增单元。
Identification of two distinct chromosome 12-derived amplification units in neuroblastoma cell line NGP.
作者信息
Van Roy N, Forus A, Myklebost O, Cheng N C, Versteeg R, Speleman F
机构信息
Department of Medical Genetics, University Hospital, Gent, Belgium.
出版信息
Cancer Genet Cytogenet. 1995 Jul 15;82(2):151-4. doi: 10.1016/0165-4608(95)00034-m.
The neuroblastoma cell line NGP contains two homogeneously staining regions (hsr). One of these hsrs contains MYCN sequences. Reverse painting experiments demonstrated that the second HSR consisted of two chromosome 12-derived amplification units, located at 12q14-15 and 12q24. Southern blot and fluorescence in situ hybridization (FISH) analysis showed amplification of genes located at 12q14-15: SAS, MDM2, and CDK4, GLI, CHOP, CDK2, and A2MR were found not to be amplified. FISH further demonstrated amplification of RSN, a gene located at 12q24. The finding of two distinct chromosome 12 amplification units in a neuroblastoma cell line NGP is reminiscent of recent findings in well-differentiated liposarcoma (WDLPS) and other sarcomas. The second amplification unit on chromosome 12 in NGP is located more distal (12q24) than the one observed in WDLPS (12q21). The mechanism and biologic significance of this amplification process in neuroblastoma and WDLPS remain to be elucidated.
神经母细胞瘤细胞系NGP包含两个均匀染色区（hsr）。其中一个hsr含有MYCN序列。反向染色体涂染实验表明，第二个HSR由两个源自12号染色体的扩增单元组成，分别位于12q14 - 15和12q24。Southern印迹和荧光原位杂交（FISH）分析显示位于12q14 - 15的基因发生了扩增：SAS、MDM2和CDK4，而GLI、CHOP、CDK2和A2MR未发现扩增。FISH进一步证明位于12q24的RSN基因发生了扩增。在神经母细胞瘤细胞系NGP中发现两个不同的12号染色体扩增单元，这让人联想到在高分化脂肪肉瘤（WDLPS）和其他肉瘤中的最新发现。NGP中12号染色体上的第二个扩增单元比在WDLPS中观察到的（12q21）位置更靠远端（12q24）。神经母细胞瘤和WDLPS中这种扩增过程的机制和生物学意义仍有待阐明。
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