Cole T J
Division Molecular Biology of the Cell I, German Cancer Research Center, Heidelberg.
Endocrinology. 1995 Oct;136(10):4693-6. doi: 10.1210/endo.136.10.7664690.
11 beta-hydroxysteroid dehydrogenase 2 (11 beta-HSD2) protects mineralocorticoid receptors from high circulating levels of glucocorticoids and cDNAs for 11 beta-HSD2 from human and sheep have been recently cloned. We have isolated the gene for mouse 11 beta-HSD2, which spans 7 kilobases and is composed of 5 exons. In comparison to the widely expressed 11 beta-HSD1, its expression is highly restricted and was detected in RNA from adult kidney, colon and small intestine only, and in contrast to sheep was not expressed in the adrenal gland. On in situ hybridization, expression of 11 beta-HSD2 was localized to the distal convoluted tubules and collecting ducts of the kidney, the same compartments of high expression of the mineralocorticoid receptor. 11 beta-HSD2 therefore appears to act in a protective role to exclude glucocorticoids from mineralocorticoid receptors in the kidney.
11β-羟类固醇脱氢酶2（11β-HSD2）可保护盐皮质激素受体免受循环中高浓度糖皮质激素的影响，并且最近已经克隆出了人类和绵羊的11β-HSD2的cDNA。我们已经分离出小鼠11β-HSD2基因，该基因跨度为7千碱基，由5个外显子组成。与广泛表达的11β-HSD1相比，其表达受到高度限制，仅在成年肾脏、结肠和小肠的RNA中检测到，并且与绵羊不同，在肾上腺中不表达。原位杂交显示，11β-HSD2的表达定位于肾脏的远曲小管和集合管，这也是盐皮质激素受体高表达的相同区域。因此，11β-HSD2似乎起到了保护作用，可将糖皮质激素排除在肾脏中的盐皮质激素受体之外。
