Doucet-Brutin S, Renoir M, Le Gallic L, Vincent S, Marty L, Fort P
Institut de Génétique Moléculaire UMR9942 CNRS, Universités Montpellier I et II BP5051, France.
Exp Cell Res. 1995 Sep;220(1):152-60. doi: 10.1006/excr.1995.1301.
Expression of many primary response cellular genes is observed during the early stages of transition from a resting G0 state to DNA synthesis. To identify gene products implicated in long-term response to growth factors, we have isolated genes sequentially activated several hours after serum stimulation of fibroblastic CCL39 cells. We report here the characterization of one of them as encoding the immunophilin of 56-59 kDa (FKBP-59), a component of the steroid receptor complex. FKBP-59 is encoded in several mammalian species by a unique gene located within the transition protein 2 gene locus. FKBP-59 mRNA is ubiquitously expressed at various levels in human organs. FKBP-59 gene activation in CCL39 fibroblasts requires protein synthesis during the first 2 h of stimulation, a period of time during which proto-oncogenes such as c-fos or c-jun are expressed. FKBP-59 mRNA degrades upon serum withdrawal and accumulates in proportion to the mitogenic strength of various purified growth factors. Its expression is reduced in CCL39 mutant-transformed cells or cells able to grow in low serum-containing medium, suggesting that FKBP-59 might participate in the negative feedback control to cell proliferation.
在从静止的G0状态转变为DNA合成的早期阶段，可以观察到许多初级反应细胞基因的表达。为了鉴定与对生长因子的长期反应有关的基因产物，我们分离了在成纤维细胞CCL39细胞受到血清刺激数小时后被依次激活的基因。我们在此报告其中一个基因的特征，它编码56 - 59 kDa的免疫亲和素（FKBP - 59），这是类固醇受体复合物的一个组成部分。FKBP - 59在几种哺乳动物物种中由位于过渡蛋白2基因座内的一个独特基因编码。FKBP - 59 mRNA在人体器官中以不同水平普遍表达。CCL39成纤维细胞中FKBP - 59基因的激活在刺激的前2小时需要蛋白质合成，这段时间原癌基因如c - fos或c - jun会表达。血清撤除后FKBP - 59 mRNA会降解，并与各种纯化生长因子的促有丝分裂强度成比例积累。其在CCL39突变转化细胞或能够在低血清培养基中生长的细胞中表达降低，这表明FKBP - 59可能参与对细胞增殖的负反馈控制。
