De Luca C, Passi S, Fabbri A A, Fanelli C
Istituto S. Gallicano (I.F.O.), Roma, Italy.
Food Addit Contam. 1995 May-Jun;12(3):445-50. doi: 10.1080/02652039509374328.
The addition of compounds able to peroxidize cell lipids (carbon tetrachloride (CCl4), cumene hydroperoxide (CUH), or linoleic acid hydroperoxide (LAH)) to 5-day-old Czapek-Dox Medium cultures of Aspergillus parasiticus induces a significant reduction of the tri-unsaturated ergosterol (ERG) levels in fungal microsomes and mitochondria, whereas the concentrations of the di-unsaturated linoleic acid (LA; C18:2 n-6) are unaffected. Aflatoxin (AFT) output follows ERG reduction and is associated with both a renewal of fungal growth and a slow increase of ERG concentration in subcellular membranes. We suggest that, by analogy with the regulatory role played on cell proliferation and metabolism by polyunsaturated fatty acid by-products (eicosanoids) in mammalian membranes, by-products of ERG oxidation may be considered triggers sufficient to induce both further fungal growth and AFT biosynthesis.
向寄生曲霉的5日龄查氏培养基培养物中添加能够过氧化细胞脂质的化合物（四氯化碳（CCl4）、氢过氧化异丙苯（CUH）或氢过氧化亚油酸（LAH）），会导致真菌微粒体和线粒体中三不饱和麦角固醇（ERG）水平显著降低，而二不饱和亚油酸（LA；C18:2 n-6）的浓度不受影响。黄曲霉毒素（AFT）产量随ERG减少而变化，并且与真菌生长的恢复以及亚细胞膜中ERG浓度的缓慢增加有关。我们认为，类似于多不饱和脂肪酸副产物（类二十烷酸）在哺乳动物膜中对细胞增殖和代谢所起的调节作用，ERG氧化的副产物可能被视为足以诱导真菌进一步生长和AFT生物合成的触发因素。
