寄生曲霉和黄曲霉纯培养物及混合培养物中黄曲霉毒素B1、B2、G1和G2的产生

Production of aflatoxins B1, B2, G1, and G2 in pure and mixed cultures of Aspergillus parasiticus and Aspergillus flavus.

作者信息

Wilson D M, King J K

机构信息

Department of Plant Pathology, University of Georgia, Tifton 31793, USA.

出版信息

Food Addit Contam. 1995 May-Jun;12(3):521-5. doi: 10.1080/02652039509374339.

DOI:10.1080/02652039509374339

PMID:7664952

Abstract

Production of aflatoxins B1, B2, G1, and G2 in pure and mixed cultures of Aspergillus parasiticus and aspergillus flavus were compared. Differing percentages of A. parasiticus (NRRL 2999) and A. flavus (NRRL 5520) conidia were used as inoculum and allowed to grow in static liquid culture for 10 days. The pure and mixed cultures of A. parasiticus/A. flavus were extracted and the aflatoxins partially purified. High-performance liquid chromatography was then used to determine aflatoxin concentrations. The maximal decreases of G1 and G2 production occurred when the inocula went from 0 to 12.5% or 25% A. flavus. Aflatoxins G1 and G2 comprised less than 10% of the total aflatoxins when the A. parasiticus/A. flavus inocula contained greater than 25% A. flavus. Production of aflatoxins B1 and B2 increased slightly as the percentage of A. flavus in the A. parasiticus/A. flavus mixed inoculum increased. A. flavus is apparently capable of suppressing accumulation of aflatoxins G1 and G2 by A. parasiticus when these fungi are grown in mixed culture.

摘要

比较了寄生曲霉和黄曲霉纯培养物及混合培养物中产黄曲霉毒素B1、B2、G1和G2的情况。使用不同比例的寄生曲霉（NRRL 2999）和黄曲霉（NRRL 5520）分生孢子作为接种物，使其在静态液体培养中生长10天。对寄生曲霉/黄曲霉的纯培养物和混合培养物进行提取，并对黄曲霉毒素进行部分纯化。然后使用高效液相色谱法测定黄曲霉毒素浓度。当接种物中黄曲霉的比例从0增加到12.5%或25%时，G1和G2的产量降幅最大。当寄生曲霉/黄曲霉接种物中黄曲霉的比例大于25%时，黄曲霉毒素G1和G2占总黄曲霉毒素的比例不到10%。随着寄生曲霉/黄曲霉混合接种物中黄曲霉比例的增加，黄曲霉毒素B1和B2的产量略有增加。当这些真菌在混合培养中生长时，黄曲霉显然能够抑制寄生曲霉积累黄曲霉毒素G1和G2。