Mukherjee U, Chatterjee S N
Biophysics Division, Saha Institute of Nuclear Physics, Calcutta.
Indian J Biochem Biophys. 1995 Feb;32(1):32-6.
Benzoate hydroxylation test revealed that Cu(II) reacting with H2O2 produced OH degree radicals, which nicked or damaged DNA or hydroxylated benzoate, the extent of damage or hydroxylation depending on the period of incubation. The production of OH degree free radicals was also supported by the scavenger studies. Neither Cu(II) nor H2O2 alone could damage DNA or hydroxylate benzoate. EDTA-chelated Cu(II) plus H2O2 could damage DNA or hydroxylate benzoate only in presence of the biological reductant, L-cysteine, the damage increased with the increasing molar ratio of L-cysteine to Cu-EDTA. The biological relevance of the EDTA chelated Cu(II) and H2O2 system is discussed.
苯甲酸盐羟基化试验表明，铜（II）与过氧化氢反应生成羟基自由基，这些自由基会切割或损伤DNA或使苯甲酸盐羟基化，损伤或羟基化的程度取决于孵育时间。清除剂研究也支持了羟基自由基的产生。单独的铜（II）或过氧化氢都不能损伤DNA或使苯甲酸盐羟基化。EDTA螯合的铜（II）加过氧化氢仅在生物还原剂L-半胱氨酸存在时才能损伤DNA或使苯甲酸盐羟基化，损伤程度随L-半胱氨酸与铜-EDTA摩尔比的增加而增加。本文讨论了EDTA螯合的铜（II）和过氧化氢体系的生物学意义。
