Yamaguchi H
Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
Nihon Geka Gakkai Zasshi. 1995 Jun;96(6):370-8.
Normal peripheral blood lymphocyte (PBL) from healthy donors, and splenic cells and tissues from patients with gastric cancers were implanted into the severe combined immunodeficient (SCID) mouse. The normal PBLs at 10(7) and 10(8)/mouse were implanted intraperitoneally (ip), and three to six splenic tissues with a size of 3 x 3 x 3 mm from gastric cancer patients were inoculated subcutaneously (sc) into the bilateral backs of the mice. The dissociated splenic cells were also administered ip and intravenously (iv). At 2, 4, 6 and 8 weeks after inoculation, mice were killed, and the human IgG and IgM were assessed by ELISA method. SCID mice with splenic cells and tissue revealed high human IgG and IgM levels from 2 weeks after inoculation, while the IgG levels in mice treated with PBLs were limited. When the tetanus toxoid was challenged to SCID mice reconstituted with splenic tissue, the anti-tetanus IgG was observed in 10 of 43 mice, showing the human B cell functions in the mice. Although the reconstitution of T cell surface marker in SCID mice was incomplete, OKT3, OKT4 and OKT8 surface markers were successfully observed in 10% to 20% SCID mice of which splenic cells were incubated with IL-2 or anti-CD3 monoclonal antibody. This model was thought to be adequate for evaluating human immunological functions of the patients with gastric cancer in vivo.
将来自健康供体的正常外周血淋巴细胞（PBL）以及胃癌患者的脾细胞和组织植入严重联合免疫缺陷（SCID）小鼠体内。将每只小鼠10⁷和10⁸个正常PBL经腹腔内（ip）植入，将来自胃癌患者的3×3×3mm大小的三至六个脾组织皮下（sc）接种到小鼠双侧背部。解离的脾细胞也经腹腔内和静脉内（iv）给药。接种后2、4、6和8周，处死小鼠，通过ELISA法评估人IgG和IgM。植入脾细胞和组织的SCID小鼠接种后2周起即显示出高人IgG和IgM水平，而用PBL处理的小鼠中的IgG水平有限。当用破伤风类毒素攻击用脾组织重建的SCID小鼠时，43只小鼠中有10只观察到抗破伤风IgG，表明小鼠中人B细胞功能。尽管SCID小鼠中T细胞表面标志物的重建不完全，但在10%至20%用IL-2或抗CD3单克隆抗体孵育脾细胞的SCID小鼠中成功观察到OKT3、OKT4和OKT8表面标志物。该模型被认为足以在体内评估胃癌患者的人免疫功能。
