Lhoste E F, Gueugneau A M, Garofano A, Philippe C, Levenez F, Corring T
Unité d'Ecologie et de Physiologie du Système Digestif, INRA Domaine de Vilvert, Jouy-en-Josas, France.
Pancreas. 1995 Jul;11(1):86-94. doi: 10.1097/00006676-199507000-00009.
The effect of cholecystokinin (CCK) on the pancreas was investigated in the pig in two experiments. Fifteen pigs were fed a diet containing 17 or 48% protein with or without MK329 (4.5 mg per meal). MK329 enhanced the postprandial peak of plasma CCK during the first 30 min, but pancreas adaptation to high protein was not affected. Sixteen pigs were divided into two groups: 12 pigs were infused with CCK-8 + secretin for 1 h and four pigs received a standard meal. In both groups, pancreatic secretion tests were performed under infusion of the vehicle alone or with MK329. After CCK + secretin, MK329 (65-500 micrograms/kg/h) did not alter CCK plasma levels and reduced the early pancreatic protein response by about 30%. Enzyme outputs in pancreatic juice were modestly affected by MK329. After the meal, MK329 (500 micrograms/kg/h) doubled the postprandial peak of plasma CCK and lowered the pancreatic protein output by 35-40% for the first 30 min. We suggest that (a) pancreatic adaptation to high dietary protein is not mediated via CCK-A receptors and (b) the stimulation of pancreatic protein secretion by a meal or by exogenous CCK-8 is mediated partly by CCK-A receptors.
在两项实验中研究了胆囊收缩素（CCK）对猪胰腺的影响。15头猪喂食含17%或48%蛋白质的日粮，添加或不添加MK329（每餐4.5毫克）。MK329在最初30分钟内增强了餐后血浆CCK峰值，但胰腺对高蛋白的适应性未受影响。16头猪分为两组：12头猪输注CCK - 8 +促胰液素1小时，4头猪进食标准餐。两组均在单独输注溶媒或与MK329一起输注的情况下进行胰腺分泌试验。在CCK +促胰液素之后，MK329（65 - 500微克/千克/小时）未改变血浆CCK水平，并使胰腺早期蛋白质反应降低约30%。胰液中的酶输出受MK329的影响较小。进食后，MK329（500微克/千克/小时）使餐后血浆CCK峰值加倍，并在最初30分钟内使胰腺蛋白质输出降低35 - 40%。我们认为：（a）胰腺对高膳食蛋白质的适应性不是通过CCK - A受体介导的；（b）进食或外源性CCK - 8对胰腺蛋白质分泌的刺激部分是由CCK - A受体介导的。
