使用时间分辨荧光检测的超灵敏生物分析测定法。
Ultrasensitive bioanalytical assays using time-resolved fluorescence detection.
作者信息
Dickson E F, Pollak A, Diamandis E P
机构信息
Department of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, Ontario.
出版信息
Pharmacol Ther. 1995 May;66(2):207-35. doi: 10.1016/0163-7258(94)00078-h.
This article reviews the use of time-resolved fluorimetric detection of lanthanide chelate luminescence as a detection method for ultrasensitive bioanalytical assays. Assay formats and detection methods, and the principle of time-resolved fluorimetric detection, are described. Detection systems, assay formats, reagents, and instrumentation for time-resolved fluorimetric detection are outlined. A review of published and commercially available immunoassays and DNA hybridization assays using time-resolved fluorimetric detection of lanthanide chelate luminescence is given.
本文综述了镧系螯合物发光的时间分辨荧光检测作为超灵敏生物分析检测方法的应用。文中描述了检测形式和检测方法，以及时间分辨荧光检测的原理。概述了时间分辨荧光检测的检测系统、检测形式、试剂和仪器。对已发表的和商业上可获得的使用镧系螯合物发光时间分辨荧光检测的免疫分析和DNA杂交分析进行了综述。
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