Sawabe K, Makiya K
Department of Parasitology and Tropical Public Health, University of Occupational and Environmental Health, Kitakyushu, Japan.
Southeast Asian J Trop Med Public Health. 1994 Dec;25(4):728-36.
The genetic difference between Angiostrongylus malaysiensis and A. cantonensis was assayed by electrophoretic analysis of isozymes. Six enzymes were analyzed using 5% polyacrylamide gel electrophoresis. Seven of 10 loci, namely GPI-1, GPI-2, HK-1, HK-2, MDH-1, MDH-2 and PGM-2, were shown to be polymorphic, but the remaining 3 loci, LDH, ME and PGM-1, were not. Both A. malaysiensis and A. cantonensis were polymorphic at 6 of the loci (p = 0.600) with heterozygosity H of 0.286 and 0.151, respectively. The Nei's genetic distance (D) between A. malaysiensis and A. cantonensis was 0.27470. This value indicates the level of interspecific variation within a genus. Through isozyme analysis, the present study demonstrated that A. malaysiensis of Japan is a valid species, separate from A. cantonensis.
通过对同工酶进行电泳分析，测定了马来西亚管圆线虫和广州管圆线虫之间的遗传差异。使用5%聚丙烯酰胺凝胶电泳分析了六种酶。10个基因座中的7个，即GPI-1、GPI-2、HK-1、HK-2、MDH-1、MDH-2和PGM-2，显示为多态性，但其余3个基因座LDH、ME和PGM-1并非如此。马来西亚管圆线虫和广州管圆线虫在6个基因座上均为多态性（p = 0.600），杂合度H分别为0.286和0.151。马来西亚管圆线虫和广州管圆线虫之间的奈氏遗传距离（D）为0.27470。该值表明了一个属内种间变异的水平。通过同工酶分析，本研究证明日本的马来西亚管圆线虫是一个有效的物种，与广州管圆线虫不同。
