Lucas J J, Hen R
Center for Neurobiology and Behavior, Columbia University, New York, NY 10032, USA.
Trends Pharmacol Sci. 1995 Jul;16(7):246-52. doi: 10.1016/s0165-6147(00)89034-3.
In recent years, cloning has revealed the existence of no less than 14 mammalian 5-HT receptors, as well as evidence that isoforms of the 5-HT4 and 5-HT7 receptors are generated by alternative splicing. In addition, molecular biology techniques have provided new tools with which to study the function of 5-HT receptors. For example, new technologies are emerging that will allow the generation of either inducible or tissue-specific knockouts (or both). In this review José Lucas and René Hen focus on the characteristics of the most recently cloned receptors and the contribution of molecular biology to the understanding of 5-HT receptor function.
近年来，克隆技术已揭示出至少14种哺乳动物5-羟色胺（5-HT）受体的存在，同时也有证据表明5-HT4和5-HT7受体的亚型是通过可变剪接产生的。此外，分子生物学技术为研究5-HT受体的功能提供了新工具。例如，新出现的技术将能够产生可诱导的或组织特异性的基因敲除动物（或两者兼具）。在这篇综述中，何塞·卢卡斯和勒内·亨重点介绍了最近克隆的受体的特性以及分子生物学在理解5-HT受体功能方面所做的贡献。
