D'Silva C G, Archibald F S, Niven D F
Department of Natural Resource Sciences, McGill University, Ste. Anne de Bellevue, Qué., Canada.
Vet Microbiol. 1995 Apr;44(1):11-23. doi: 10.1016/0378-1135(94)00109-a.
Four strains of the swine pathogen, Actinobacillus pleuropneumoniae, namely, the type strain (ATCC 27088; biotype 1), the 'reference' strain of biotype 2 (Bertschinger 2008/76), and two additional biotype 1 strains, strain BC181, which is less virulent than the type strain, and strain K17, which was isolated from a lamb, were investigated with respect to iron acquisition. All strains produced iron-repressible outer membrane proteins. However, only the type and biotype 2 strains could acquire iron from porcine transferrin and no organism could utilize human, bovine or ovine transferrin, or ovine or porcine lactoferrin; haemoglobin supported good growth of all strains except strain K17. In all cases, iron acquisition from transferrin and haemoglobin required direct contact between the organisms and the proteins indicating the existence of specific receptors. An affinity isolation technique, using biotinylated porcine transferrin plus streptavidin-agarose, allowed the isolation of the following polypeptides from total membranes of organisms grown under iron-restricted conditions: 99 kDa and 64 kDa from ATCC 27088; 93 kDa from Bertschinger 2008/76; 95 kDa (trace amounts) and 60 kDa from BC181; none from K17. These results indicate that the 93-99 kDa polypeptides are involved in the acquisition of iron from porcine transferrin and that the inability of strain K17 to use transferrin as an iron source is due, probably, to the lack of, or a defect in, an analogous component.
对猪胸膜肺炎放线杆菌的四株菌株进行了铁摄取方面的研究，这四株菌株分别为：模式菌株（ATCC 27088；生物型1）、生物型2的“参考”菌株（Bertschinger 2008/76），以及另外两株生物型1菌株，其中BC181菌株的毒力低于模式菌株，K17菌株是从一只羔羊中分离出来的。所有菌株都产生铁抑制性外膜蛋白。然而，只有模式菌株和生物型2菌株能够从猪转铁蛋白中获取铁，没有菌株能够利用人、牛或羊的转铁蛋白，或羊或猪的乳铁蛋白；血红蛋白支持除K17菌株外所有菌株的良好生长。在所有情况下，从转铁蛋白和血红蛋白中获取铁需要生物体与蛋白质直接接触，这表明存在特异性受体。一种亲和分离技术，使用生物素化的猪转铁蛋白加链霉亲和素琼脂糖，能够从在铁限制条件下生长的生物体的总膜中分离出以下多肽：来自ATCC 27088的99 kDa和64 kDa；来自Bertschinger 2008/76的93 kDa；来自BC181的95 kDa（痕量）和60 kDa；来自K17的无。这些结果表明，93 - 99 kDa的多肽参与了从猪转铁蛋白中获取铁的过程，并且K17菌株无法将转铁蛋白用作铁源可能是由于缺乏类似成分或该成分存在缺陷。
