STA葡糖淀粉酶基因的S1、S2和SGA1祖先基因均定位于酿酒酵母的IX号染色体上。

The S1, S2 and SGA1 ancestral genes for the STA glucoamylase genes all map to chromosome IX in Saccharomyces cerevisiae.

作者信息

Lambrechts M G, Pretorius I S, Marmur J, Sollitti P

机构信息

Department of Microbiology, University of Stellenbosch, South Africa.

出版信息

Yeast. 1995 Jun 30;11(8):783-7. doi: 10.1002/yea.320110810.

DOI:10.1002/yea.320110810

PMID:7668048

Abstract

The polymorphic extracellular glucoamylase-encoding genes STA1 (chr. IV), STA2 (chr. II) and STA3 (chr. XIV), from Saccharomyces cerevisiae var. diastaticus probably evolved by genomic rearrangement of DNA regions (S1, S2 and SGA1) present in S. cerevisiae, and subsequent translocation to unlinked regions of chromosomal regions. S1, encoding a homologue to the threonine/serine-rich domain of STA glucoamylases (GAI-III), mapped to the right arm of chromosome IX. S2, encoding the hydrophobic leader peptide of GAI-III), was also mapped on the right arm of chromosome IX, next to S1, close to DAL81. The SGA1 sporulation-specific, intracellular glucoamylase-encoding gene is located on the left arm of chromosome IX, 32 kb proximal of HIS5.

摘要

来自糖化酵母变种的多态性细胞外葡糖淀粉酶编码基因STA1（第四条染色体）、STA2（第二条染色体）和STA3（第十四条染色体）可能是通过酿酒酵母中存在的DNA区域（S1、S2和SGA1）的基因组重排，随后易位到染色体区域的不连锁区域而进化而来。S1编码STA葡糖淀粉酶（GAI-III）富含苏氨酸/丝氨酸结构域的同源物，定位于第九条染色体的右臂。S2编码GAI-III的疏水前导肽，也定位于第九条染色体的右臂，紧挨着S1，靠近DAL81。SGA1孢子形成特异性细胞内葡糖淀粉酶编码基因位于第九条染色体的左臂，在HIS5近端32 kb处。