Ribeiro-Paes J T, Rodrigues V
Departamento de Parasitologia, Microbiologia e Immunologia, Faculdade de Medicina de Ribeirão Preto, Brazil.
Comp Biochem Physiol B Biochem Mol Biol. 1995 May;111(1):69-74. doi: 10.1016/0305-0491(94)00231-i.
The phenol oxidase system, which is thought to play a central role in eggshell formation, was analyzed by means of electrophoretic and histochemical techniques. In contrast to current beliefs, our data show that males also express the phenol oxidase activity. The analysis of the electrophoretic pattern from males and females shows differences: adult males present a major band and a faint band, migrating slightly below. Adult females express a more complex pattern composed of four bands. Histochemical studies revealed that female phenol oxidase is concentrated in the vitelline cells, is inhibited by DDC, and this inhibition is correlated with disruption of female egg production. The present studies, in agreement with other reports, reveal that female phenol oxidase may be involved in sclerotization of the S. mansoni eggshell.
酚氧化酶系统被认为在蛋壳形成过程中起核心作用，通过电泳和组织化学技术对其进行了分析。与当前观点相反，我们的数据表明雄性也表达酚氧化酶活性。对雄性和雌性电泳图谱的分析显示出差异：成年雄性呈现一条主带和一条微弱的带，迁移位置略低。成年雌性表达由四条带组成的更复杂的图谱。组织化学研究表明，雌性酚氧化酶集中在卵黄细胞中，被二硫苏糖醇（DDC）抑制，并且这种抑制与雌性产卵的破坏相关。本研究与其他报告一致，表明雌性酚氧化酶可能参与曼氏血吸虫蛋壳的硬化过程。
