Canaux S, Foulquier F, Duprat A M, Moreau M
Centre de Biologie du Développement, UMR 9925 CNRS, Université Paul Sabatier, affiliée à l'INSERM, Toulouse, France.
Int J Dev Biol. 1995 Apr;39(2):327-33.
Changes in activity of the Na+, K+ ATPase of maturing Pleurodeles waltl were followed by measuring the resting potential in presence or absence of the specific inhibitor dihydroouabain. Corresponding currents were measured in voltage clamp conditions to eliminate the differences in resting potential at the origin and at the end of the meiotic maturation process. Our data confirm previous results obtained on Xenopus, indicating that the Na+,K+ pump activity disappears from the plasma membrane during progesterone-induced maturation and can be reactivated by an increase in internal Ca2+ triggered by ionomycin. Moreover we show by ultrastructural histochemistry that these modulations are likely to depend on the internalization and reinsertion of the transporter into the plasma membrane.
通过在存在或不存在特异性抑制剂二氢哇巴因的情况下测量静息电位，追踪成熟的疣螈（Pleurodeles waltl）中Na⁺、K⁺ -ATP酶活性的变化。在电压钳制条件下测量相应的电流，以消除减数分裂成熟过程开始和结束时静息电位的差异。我们的数据证实了先前在非洲爪蟾上获得的结果，表明在孕酮诱导的成熟过程中，Na⁺、K⁺泵活性从质膜上消失，并且可以通过离子霉素引发的细胞内Ca²⁺增加而重新激活。此外，我们通过超微结构组织化学表明，这些调节可能取决于转运体内化并重新插入质膜。
