Gallo P, Saviano M, Rossi F, Pavone V, Pedone C, Ragone R, Stiuso P, Colonna G
Centro Interdipartimentale di Ricerca sui Peptidi Bioattivi and CEINGE-Biotecnologie, Napoli, Italia.
Biopolymers. 1995 Sep;36(3):273-81. doi: 10.1002/bip.360360303.
Cyclophilin A, a peptidyl-prolyl cis-trans is isomerase that catalyzes the otherwise slow isomerization of Xaa-Pro imidic bond, specifically binds the immunosuppressant cyclosporin A. Herein we report evidence on binding of cyclolinopeptide A and its synthetic analogue, [Aib5,6-D-Ala8]cyclolinopeptide, to bovine cyclophilin A. Binding experiments were monitored by fluorescence, CD, and second-derivative spectroscopies, evidencing no remarkable rearrangement of protein structure organization. The possibility that cyclolinopeptide A could act as a substitute of cyclosporin A in the immunosuppression modulation is also briefly discussed.
亲环蛋白A是一种肽基脯氨酰顺反异构酶，可催化Xaa-Pro亚胺键原本缓慢的异构化反应，它能特异性结合免疫抑制剂环孢菌素A。在此我们报告亲环素环肽A及其合成类似物[Aib5,6-D-Ala8]亲环素环肽与牛亲环蛋白A结合的证据。通过荧光、圆二色光谱和二阶导数光谱监测结合实验，结果表明蛋白质结构组织没有明显重排。本文还简要讨论了亲环素环肽A在免疫抑制调节中替代环孢菌素A的可能性。
