Barrio Rendo M E
Department of Physiology, University of Buenos Aires School of Medicine, Argentina.
In Vivo. 1995 Jan-Feb;9(1):71-3.
The erythroid response to Erythropoietin (EPO) is highly dependent on dietary protein. Its operating mechanism, however, remains unsettled. The mouse spleen is an erythropoietic organ which contains an EPO-responsive cell population that can be easily amplified by administration of the hormone. The present study was performed to determine the effect of a protein-free diet offered freely to mice up to two days after injection of r-Hu EPO (1000mU/200 ul) on the response of the above population. Splenic cell suspensions from control and experimental mice were prepared in microwells containing 400 mU r-Hu EPO and appropriate medium. The response to EPO was evaluated in terms of 3H-thymidine uptake. The results obtained indicate that acutely induced protein restriction suppressed the response of the EPO-responsive splenic cell population to EPO when it was imposed on mice immediately after hormone injection, and suggest the appearance of deficient rates of differentiation of erythropoietic units by protein restriction.
红细胞对促红细胞生成素（EPO）的反应高度依赖于膳食蛋白质。然而，其作用机制仍未明确。小鼠脾脏是一个造血器官，其中含有对EPO有反应的细胞群体，通过给予该激素可使其容易扩增。本研究旨在确定在注射重组人促红细胞生成素（r-Hu EPO，1000mU/200μl）后长达两天内，自由给予小鼠无蛋白饮食对上述细胞群体反应的影响。在含有400mU r-Hu EPO和适当培养基的微孔中制备对照小鼠和实验小鼠的脾细胞悬液。根据3H-胸腺嘧啶核苷摄取来评估对EPO的反应。所得结果表明，在激素注射后立即对小鼠施加急性诱导的蛋白质限制会抑制对EPO有反应的脾细胞群体对EPO的反应，并提示蛋白质限制导致造血单位分化率不足。
