Glover R P, Sweetman G M, Farmer P B, Roberts G C
Centre for Mechanisms of Human Toxicity, University of Leicester, UK.
Rapid Commun Mass Spectrom. 1995;9(10):897-901. doi: 10.1002/rcm.1290091007.
We report the sequence analysis of an oligodeoxyribonucleotide by electrospray mass spectrometry (ES-MS). For full sequence analysis of the oligomer, d(CGTAGCTACG), an enzyme digestion step followed by off-line high performance liquid chromatography was used. For ES-MS analysis, introducing the sample in an imidazole buffer with 70% acetonitrile increased the electrospray ion currents and helped reduce sodium-adduct ion peaks.
我们报道了通过电喷雾质谱法（ES-MS）对寡脱氧核糖核苷酸进行的序列分析。对于寡聚物d（CGTAGCTACG）的全序列分析，采用了酶消化步骤，随后进行离线高效液相色谱分析。对于ES-MS分析，将样品引入含70%乙腈的咪唑缓冲液中可增加电喷雾离子电流，并有助于减少钠加合物离子峰。
